Supplemental Figures for "FoxP2 Expression in Avian Vocal Learners and Non-Learners" J. Neurosci. Haesler et al. 24: 3164 (2004)

  • Supplemental Figure 1. Sequence comparison of the zfFoxP2 and zfFoxP1 proteins. A, Alignment of zfFoxP2 (isoform I), mouse Foxp2 (accession NP_444472.2), and human FOXP2 (AAN03408.1). In the mouse and human sequence, only amino acids that differ from the zebra finch sequence are shown. Protein motifs are highlighted as in Figure 1A. In the human sequence, the R553H mutation that is associated with the speech and language disorder is marked with an asterisk, a primate-specific amino acid is boxed, a carnivore-specific amino acid is circled, and the unique human-specific amino acid is highlighted by a triangle. B, Sequence comparison of zfFoxP1 with zfFoxP2. Identical amino acids are shaded dark gray; similar amino acids are shaded light gray.

  • Supplemental Figure 2. In contrast to developmental differences in the ratio of FoxP2 mRNA expression of area X to the surrounding striatum, adjacent sections showed that no such differences existed when tested for the expression of the glutamate receptor subunit NR2B (A) and subtype mGluR2 (B) during development of zebra finches (n = 3 animals per data point).

  • Supplemental Figure 3. FoxP2 expression is not induced in area X by singing. In both young (PHD 65, B, D) and adult (PHD 150, F, H) zebra finches, singing undirected song does not induce FoxP2 expression in area X, whereas in adjacent sections of the same animals, the amount of ZENK expressed reflects the singing activity during the last 30 min before the birds were killed (A, C, E, G). In adult canaries, there was also no relationship between the amount the bird sang before being killed and the amount of FoxP2 expression in area X (I--O).

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